Related Experiment Video
Updated: Feb 12, 2026

Measurement of Tissue Non-Heme Iron Content using a Bathophenanthroline-Based Colorimetric Assay
Published on: January 31, 2022
[Participation of sinusoidal elements in experimental iron overload. Ultrastructural and microanalytic study]
Experimental iron overload reveals iron particles within Kupffer and Ito cells. This confirms the microphagocytic role of Ito cells in iron processing.
Area of Science:
- Hepatology
- Cell Biology
- Toxicology
Context:
- Investigating cellular mechanisms of iron metabolism.
- Studying the effects of experimental iron overload in liver tissue.
Purpose:
- To elucidate the role of sinusoidal cells in iron homeostasis.
- To identify the cellular localization and characteristics of iron deposits.
Summary:
- Ultrastructural and microanalytical studies identified iron particles in Kupffer and Ito cells.
- The presence of iron in Ito cells indicates their microphagocytic function.
- This research clarifies cellular responses to iron overload.
Impact:
- Provides insights into the cellular handling of excess iron in the liver.
- Highlights the functional capacity of Ito cells beyond their known roles.
- Contributes to understanding the pathogenesis of iron-loading disorders.
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