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Effect of cyclophosphamide on mouse bone marrow and leukemic stem cells
Archivum Immunologiae Et Therapiae Experimentalis
|January 1, 1980
Summary
The colony forming unit assay differentiates normal and leukemic stem cells. Cyclophosphamide (CY) demonstrated varying effects on normal bone marrow stem cells versus leukemic stem cells in vivo.
Area of Science:
- Oncology
- Hematology
- Pharmacology
Background:
- The colony forming unit (CFU) assay is crucial for evaluating antitumor agents.
- Understanding differential effects on normal and cancerous stem cells is vital for treatment development.
Purpose of the Study:
- To introduce and validate the colony forming unit assay for in vivo studies.
- To assess the differential sensitivity of normal and leukemic stem cells to an antitumor agent.
Main Methods:
- The colony forming unit assay was employed in vivo.
- Cyclophosphamide (CY) was used to test the assay's efficacy.
- Effects on normal bone marrow stem cells (NCFU) and leukemic stem cells (LCFU) were quantified.
Main Results:
- The colony forming unit assay successfully differentiated between normal and leukemic stem cells.
- Cyclophosphamide (CY) exhibited differential sensitivity between normal mouse bone marrow stem cells and P-388 leukemic stem cells.
- The assay demonstrated the varying impact of CY on stem cell populations.
Conclusions:
- The colony forming unit assay is a valuable tool for in vivo assessment of antitumor agents.
- Differential stem cell sensitivity to chemotherapy can be effectively evaluated using this method.
- This technique aids in understanding drug effects on both normal and malignant hematopoiesis.