The effect of displacement from protein binding on serum and tissue fluid levels of cephazolin

Displacement of cephazolin, a highly protein-bound beta-lactam antibiotic, by sulpha-phenazole was studied in vitro and in vivo. In vitro, the sulphonamide was effective in displacing the antibiotic from its binding sites at the concentration 100--1000 mumol 1(-1). In vivo, both the serum and the tissue fluid concentration of free cephazolin increased significantly in rats concomitantly treated with sulphaphenazole.