Estradiol receptor activity in lyophilized calf uterus and human breast tumor tissue

T Benraad , A Koenders

Cancer +

|

December 15, 1980

View abstract on PubMed

Summary

Lyophilized human breast tumor and calf uterus tissues show stable estradiol receptors (ER) during storage. This stability supports using these lyophilized tissues for reliable quality control in steroid receptor measurements.

Area Of Science

Biochemistry
Oncology
Endocrinology

Background

Estradiol receptors (ER) are crucial biomarkers in breast cancer diagnostics.
Accurate measurement of ER is essential for effective patient treatment.
Standardization of steroid receptor assays is needed for reliable results.

Purpose Of The Study

To evaluate the stability of estradiol receptors (ER) in lyophilized human breast tumor and calf uterus tissues.
To determine the feasibility of using lyophilized tissues for quality control in steroid receptor measurements.

Main Methods

Estradiol receptors (ER) were analyzed using the dextran-coated charcoal method.
Lyophilized human breast tumor and calf uterus tissues were stored under various conditions (refrigerated and room temperature) for extended periods.

Main Results

Lyophilized calf uterus tissue showed no decline in ER binding sites after 6-8 months at 0-4°C and 2 weeks at room temperature.
Lyophilized human breast tumor tissue exhibited no or minimal decrease in ER binding sites after several months at 0-4°C and 4 weeks at room temperature.

Conclusions

Lyophilized target tissues, including human breast tumors and calf uteri, demonstrate excellent stability for ER binding sites.
These findings support the use of lyophilized tissues as a reliable standard for both intra- and interlaboratory quality control of steroid receptor assays.