Related Experiment Video
Updated: Aug 9, 2026
11:31
Determining Optimal Cytotoxic Activity of Human Her2neu Specific CD8 T cells by Comparing the Cr51 Release Assay to the xCELLigence System
Published on: August 8, 2012
Immunochemical differences between glucocorticoid receptors from corticoid-sensitive and -resistant malignant
Cancer Research
|January 1, 1981
Summary
Antibodies against rat glucocorticoid receptors can identify similar receptors in mouse and human cancer cells. This technique differentiates between corticoid-sensitive and resistant cell types, aiding in understanding glucocorticoid receptor function.
Area of Science:
- Immunology
- Molecular Biology
- Oncology
Background:
- Glucocorticoid receptors (GRs) are crucial for cellular response to glucocorticoids.
- Studying GRs in malignant lymphocytes is vital for understanding corticoid resistance in cancer therapy.
Purpose of the Study:
- To investigate the immunochemical properties of GRs in murine and human malignant lymphocytes.
- To assess the cross-reactivity of antibodies raised against rat liver GRs with GRs from other species.
Main Methods:
- Purified immunoglobulin G against rat liver GRs was covalently linked to Sepharose CL-4B to create an immunoaffinity column.
- The affinity column's ability to recognize cytosolic [3H]triamcinolone acetonide-receptor complexes was tested using various mouse lymphoma and human leukemia cell lines.
Main Results:
- The immunoaffinity column successfully retained 70-84% of GR complexes from corticoid-sensitive (CS) mouse and human lymphocytes.
- The column failed to recognize the GRs in corticoid-resistant mouse lymphoma cells, which exhibited a different Stokes radius.
Conclusions:
- Antibodies against rat liver GRs demonstrate cross-reactivity with GRs from mouse and human malignant lymphocytes.
- This immunoaffinity approach can distinguish between CS and corticoid-resistant GRs based on size and immunoreactivity.
