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Excretion of carcinoma products in irradiated C3H/He mice
Abstract:
Local low-dose (200 rads) gamma-irradiation to both kidneys impaired the excretion of 3H-labeled tumor products and reduced the survival time of mice carrying carcinomas in the ascites form. Daily i.p. injections of cell-free ascites fluid into tumor-free mice for 3 weeks resulted in the death of 25 of 180 irradiated animals, with no deaths among 180 injected unirradiated controls. The only histologically visible effects of irradiation of the kidneys during ascites tumor growth or during i.p. injections of cell-free ascites fluid was a cloudy swelling of the tubular epithelium in the renal cortex together with excessive protein in the tubular lumens.
Insights
Low-dose gamma irradiation of kidneys in tumor-bearing mice impaired tumor product excretion and reduced survival. Irradiated mice receiving ascites fluid experienced mortality, unlike controls, indicating kidney irradiation
Area of Science:
- Oncology
- Radiation Biology
- Nephrology
Background:
- Ascites tumors can produce substances that affect host physiology.
- Kidney function is crucial for clearing metabolic byproducts.
- The impact of localized kidney irradiation on tumor-bearing hosts is not fully understood.
Purpose of the Study:
- To investigate the effects of local low-dose gamma irradiation on kidney function in mice with ascites carcinomas.
- To assess the impact of such irradiation on the excretion of tumor products and overall survival.
- To determine if ascites fluid itself poses a toxicity risk to irradiated kidneys.
Main Methods:
- Mice bearing ascites form carcinomas were subjected to local low-dose (200 rads) gamma irradiation to both kidneys.
- Excretion of 3H-labeled tumor products was monitored.
- Tumor-free mice received daily intraperitoneal injections of cell-free ascites fluid for 3 weeks, with some groups being irradiated.
- Kidney histology was examined.
Main Results:
- Kidney irradiation significantly impaired the excretion of 3H-labeled tumor products.
- Irradiated mice with ascites tumors exhibited reduced survival times.
- Intraperitoneal injection of cell-free ascites fluid led to mortality in irradiated mice but not in unirradiated controls.
- Histological examination revealed cloudy swelling of renal tubular epithelium and protein accumulation in irradiated kidneys.
Conclusions:
- Local low-dose gamma irradiation of kidneys negatively impacts tumor product excretion and survival in mice with ascites carcinomas.
- Irradiated kidneys are susceptible to damage from ascites fluid components, leading to toxicity and mortality.
- These findings highlight the critical role of kidney function in managing tumor burden and the potential adverse effects of radiation therapy on renal health.