Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

RIA opiates: structure versus reactivity

R D Budd, W J Leung, F C Yang

    Clinical Toxicology
    |October 1, 1980
    PubMed
    Summary
    This summary is machine-generated.

    Structural differences in 26 opiates were analyzed using radioimmunoassay (RIA). Key findings show the 5-ring structure is vital for reactivity, with alkyl group addition enhancing antibody affinity.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [Malignant embryonal rhabdomyosarcoma with heterologous differentiation in gastric stromal tumors: report of a case].

    Zhonghua bing li xue za zhi = Chinese journal of pathology·2026
    Same author

    [Mid-term clinical outcomes of supraspinatus tendon reconstruction versus superior capsular reconstruction for the treatment of irreparable posterosuperior massive rotator cuff tears].

    Zhonghua yi xue za zhi·2025
    Same author

    [Primary immunodeficiency disease based on ITK mutation: report of a case].

    Zhonghua bing li xue za zhi = Chinese journal of pathology·2024
    Same author

    [Efficacy analysis of surgical combined with postoperative adjuvant therapy for T3 gallbladder carcinoma: a multicenter retrospective study].

    Zhonghua wai ke za zhi [Chinese journal of surgery]·2023
    Same author

    Analysis of patient attitudes and behavior regarding dermatologic care during the COVID-19 pandemic: a survey-based study at a single academic institution.

    Dermatology online journal·2023
    Same author

    [Clinical value of extended radical resection for pT3 stage gallbladder cancer].

    Zhonghua yi xue za zhi·2020
    Same journal

    Chronic central nervous system toxicity of the chickling pea (Lathyrus sativus).

    Clinical toxicology·1981
    Same journal

    Brain energy metabolism in mice exposed to oxygen at 1 atmosphere absolute.

    Clinical toxicology·1981
    Same journal

    Toxicity of some essential plant oils. Clinical and experimental study.

    Clinical toxicology·1981
    Same journal

    Carbon disulfide neuropathy in rats. A morphological and ultrastructural study of degeneration and regeneration.

    Clinical toxicology·1981
    Same journal

    Regional differences in brain-soluble acetylcholinesterase and its molecular forms after acute poisoning by isoflurophate in rats.

    Clinical toxicology·1981
    Same journal

    Industrial neuropathy due to n-hexane. Clinical and morphological findings in three cases.

    Clinical toxicology·1981
    See all related articles

    Area of Science:

    • Pharmacology
    • Medicinal Chemistry
    • Analytical Chemistry

    Background:

    • Opiates are a class of drugs with significant medical and societal impact.
    • Understanding opiate structure-activity relationships is crucial for drug development and forensic analysis.
    • Radioimmunoassay (RIA) is a sensitive method for quantifying small molecules like opiates.

    Purpose of the Study:

    • To investigate the relationship between opiate chemical structure and binding affinity to a specific morphine antibody.
    • To determine how modifications to the morphine molecule affect its recognition by the Roche RIA morphine antibody.
    • To elucidate structure-activity relationships for a range of opiate compounds.

    Main Methods:

    • Analysis of 26 different opiate compounds.

    Related Experiment Videos

  • Utilized radioimmunoassay (RIA) for quantitative analysis.
  • Tested compounds at five varying concentrations to establish dose-response curves.

    • Main Results:

    • The fundamental 5-ring opiate core structure was confirmed as essential for antibody binding and reactivity.
    • Introduction of an alkyl group at the 3-position of the morphine molecule significantly increased binding affinity.
    • Alterations to other critical functional groups on the morphine structure resulted in decreased binding affinity.

    Conclusions:

    • Opiate structure-activity relationships are highly sensitive to specific substituent placement.
    • The 3-position modification represents a key area for enhancing opiate affinity for this particular antibody.
    • These findings contribute to a deeper understanding of opiate-antibody interactions and can inform future drug design.