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Related Experiment Videos

Immersion weighing method for recording the flows through macroscopic membranes

D G Mărgineanu, M L Flonta

    Journal of Biochemical and Biophysical Methods
    |August 1, 1980
    PubMed
    Summary

    This study presents a cost-effective method for measuring osmotic and diffusional water flows using apparent weight changes. The technique achieves high sensitivity, enabling precise quantification of water movement across membranes.

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    Area of Science:

    • Biophysics
    • Physical Chemistry
    • Environmental Science

    Background:

    • Osmotic and diffusional water flows are critical in biological and chemical systems.
    • Accurate measurement of these flows is essential for understanding transport phenomena.
    • Existing methods can be complex or expensive.

    Purpose of the Study:

    • To develop a simple, reliable, and sensitive method for measuring osmotic and diffusional water flows.
    • To establish a technique applicable to membrane-bounded systems.

    Main Methods:

    • Continuous recording of apparent weight of a membrane-bounded vessel.
    • Immersion of the vessel in solutions of varying densities.
    • Comparison of solution density inside and outside the vessel.

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    Main Results:

    • The method provides a cheap and reliable way to measure osmotic flows.
    • Achieved sensitivity of 10(-5) kg x m-2 x s-1 for water flow.
    • Demonstrated applicability to diffusional (isotopic) water flows.

    Conclusions:

    • The apparent weight recording method is a viable technique for quantifying water transport.
    • This method offers a sensitive and accessible approach for studying osmotic and diffusional processes.
    • The technique has broad applications in various scientific disciplines requiring water flow measurements.