Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Solid-phase immune electron microscopy (SPIEM) for rapid viral diagnosis

D Katz, Y Straussman, A Shahar

    Journal of Immunological Methods
    |January 1, 1980
    PubMed
    Summary

    Solid-phase immune electron microscopy (SPIEM) offers a solution to challenges in traditional immune electron microscopy (IEM) for virus detection. This method utilizes S. aureus bacteria to efficiently capture Sindbis virus for enhanced diagnosis.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Towards Multireference Equivalents of the HEAT Thermochemical Protocol.

    Journal of computational chemistry·2025
    Same author

    Experiments reveal extreme water generation during planet formation.

    Nature·2025
    Same author

    The reduction behavior of sulfurized polyacrylonitrile (SPAN) in lithium-sulfur batteries using a carbonate electrolyte: a computational study.

    Physical chemistry chemical physics : PCCP·2024
    Same author

    A generalized hybrid scheme for multireference methods.

    The Journal of chemical physics·2021
    Same author

    Neutralization of the anthrax toxin by antibody-mediated stapling of its membrane-penetrating loop.

    Acta crystallographica. Section D, Structural biology·2021
    Same author

    Azathioprine for prevention of clinical recurrence in Crohn's disease patients with severe endoscopic recurrence: an IG-IBD randomized double-blind trial.

    European review for medical and pharmacological sciences·2020

    Area of Science:

    • Virology
    • Immunology
    • Microscopy

    Background:

    • Immune electron microscopy (IEM) is a sensitive viral detection method.
    • Traditional IEM faces challenges with optimal precipitation and prozone effects.
    • Solid-phase immune electron microscopy (SPIEM) addresses these limitations.

    Purpose of the Study:

    • To introduce and evaluate the solid-phase immune electron microscopic (SPIEM) technique.
    • To demonstrate SPIEM's effectiveness in overcoming IEM limitations.
    • To report the use of S. aureus as a solid phase for Sindbis virus detection.

    Main Methods:

    • Antibodies (anti-Sindbis gamma globulins) are attached to S. aureus bacteria via protein A.
    • The antibody-coated bacteria are used to capture Sindbis virus from suspension.

    Related Experiment Videos

  • Preparations are analyzed using transmission or scanning electron microscopy after centrifugation.

    • Main Results:

    • SPIEM successfully detected Sindbis virus.
    • The virus was observed attached to the surface of the S. aureus particles in positive samples.
    • The use of S. aureus as a solid phase proved effective for Sindbis virus detection.

    Conclusions:

    • SPIEM is a viable alternative to traditional IEM, offering improved reliability.
    • The S. aureus-based SPIEM method is efficient for Sindbis virus detection.
    • This technique enhances the diagnosis and detection capabilities for viruses.