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The nature of the prostaglandin-producing mononuclear cell in human peripheral blood
Abstract:
The purpose of this investigation was to identify the human peripheral mononuclear cell responsible for PGE production. The prostaglandin-producing cell had the following characteristics: (1) was glass adherent; (2) was removed by techniques that effectively removed phagocytic cells (carbonyl iron); and (3) was not an E-rosette cell. Furthermore, the peripheral mononuclear cells that contain cytoplasmic PGE by indirect immunofluorescence also ingest latex particles. B and T lymphoid cell lines did not produce large quantities of PGE. Mononuclear cells separated on discontinuous BSA gradients varied greatly in their ability to produce prostaglandin (low density > high density). These data constitute strong evidence that the majority of PGE produced in peripheral mononuclear cell suspensions emanates from a population of low-density monocytes.
Insights
This study identifies monocytes as the primary source of prostaglandin E (PGE) in human peripheral blood. Low-density monocytes, which are glass adherent and phagocytic, are responsible for most PGE production.
Area of Science:
- Immunology
- Cell Biology
Background:
- Prostaglandin E (PGE) plays a crucial role in immune responses.
- Identifying the specific cell type responsible for PGE production in human peripheral blood is essential for understanding immune regulation.
Purpose of the Study:
- To identify the specific human peripheral mononuclear cell type responsible for Prostaglandin E (PGE) production.
Main Methods:
- Characterization of PGE-producing cells based on adherence, phagocytic removal (carbonyl iron), and E-rosette negativity.
- Indirect immunofluorescence to detect cytoplasmic PGE and latex particle ingestion.
- Separation of mononuclear cells on discontinuous BSA gradients.
Main Results:
- The prostaglandin-producing cell was glass adherent, removed by phagocytic cell removal techniques, and not an E-rosette cell.
- Peripheral mononuclear cells containing cytoplasmic PGE also ingested latex particles.
- Low-density monocytes demonstrated a significantly higher capacity for PGE production compared to high-density cells.
- B and T lymphoid cell lines produced minimal PGE.
Conclusions:
- The majority of PGE produced in human peripheral mononuclear cell suspensions originates from low-density monocytes.
- Monocytes are the primary prostaglandin-producing cells within peripheral mononuclear cell populations.