Feulgen-deoxyribonucleic acid values in the cytology of bladder cancer

The deoxyribonucleic acid values of cells separated from fixed specimens from patients with bladder cancer were determined with the use of a microspectrophotometer and were compared to the grade and stage of cancer tissues. A histogram of nuclear deoxyribonucleic acid values showed flatness of the mode, deviation of the stem line to the right and an increase of polyploid cells associated with an increase in the grade and stage of cancer. Prognosis seems to be related more closely to deoxyribonucleic acid values off cancer tissues than to the stage and grade of cancer. Deoxyribonucleic acid values of exfoliated cells from fresh cancer tissue and the cells in the urine of the same patients were compared, revealing similar patterns. Indication of grade could be made in some cases by determining nuclear deoxyribonucleic acid values of exfoliated cells in urine. This result indicates that determination of nuclear deoxyribonucleic acid values is a valuable parameter for automated evaluation of urinary cytology.