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Related Experiment Videos

Trypsin fails as Australian snake bite cure

A J Broad, S K Sutherland, K E Lovering

    The Medical Journal of Australia
    |October 4, 1980
    PubMed
    Summary

    Trypsin showed potential in inactivating snake venom lethal potency in vitro. However, premixing or delayed injection of trypsin did not improve mouse survival rates against tiger snake venom.

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    Area of Science:

    • Toxicology
    • Pharmacology
    • Biochemistry

    Background:

    • Snakebite envenoming is a significant global health issue.
    • Trypsin has been proposed as a potential treatment for venomous snake bites.
    • The efficacy of trypsin as an antidote requires rigorous scientific evaluation.

    Purpose of the Study:

    • To investigate the effectiveness of trypsin in neutralizing snake venom.
    • To determine if premixing or delayed administration of trypsin improves survival rates in a mouse model.

    Main Methods:

    • In vitro incubation of tiger snake venom (TSV) with trypsin.
    • Injection of venom-trypsin mixtures or sequential injections into mice.
    • Comparison of survival rates between treatment groups and controls.
    • Evaluation of antivenom efficacy with delayed administration.

    Main Results:

    • Significant inactivation of TSV lethal potency was observed in vitro after trypsin incubation.
    • Premixing TSV and trypsin before injection did not improve mouse survival.
    • Administering trypsin 10-30 minutes after TSV injection did not increase survival rates.
    • Specific antivenom remained effective even with a 10-minute delay post-venom injection.
    • Different venoms exhibited varying susceptibility to trypsin inactivation in vivo.

    Conclusions:

    • While trypsin can inactivate snake venom in vitro, its therapeutic efficacy in vivo for snakebite treatment is limited.
    • Early administration of specific antivenom is crucial for effective treatment of envenomation.
    • Further research is needed to explore the variable inactivation of different venoms by trypsin.

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