Element analysis of skeletal muscle in Duchenne muscular dystrophy using x-ray fluorescence spectrometry

Concentrations of calcium, iron, copper, and zinc were simultaneously measured by x-ray fluorescence spectrometry in muscle biopsies from 11 normal subjects, 13 patients with Duchenne muscular dystrophy (DMD), and 3 DMD carriers. Calcium and copper levels were significantly elevated in patients with DMD compared to controls, and one presumptive carrier also showed an elevated calcium concentration. Comparisons with morphological data for the same samples showed a significant positive correlation between calcium concentration and the percentage area of type 2 fibers in controls, but no other correlations were found to be significant. These results illustrate the value of x-ray fluorescence spectrometry and provide further evidence for the involvement of calcium in dystrophic processes.