[Structural organization of the genome of bacteriophage T5 by specific endonucleases]

The DNA of Bacteriophage T5+ has been treated with restriction endonucleases EcoRI, HindIII, BamI, SmaI, PstI, SalI, KpnI and the electrophorectic pattern obtained in agarose gel has been analyzed in order to localize the specific cleavage sites on the T5 DNA. The localization of cleavage sites has been resolved from the electrophoretic pattern of double and partial digests, the digests of isolated restriction fragments and the digests of deletion mutant T5 st(O) DNA. Four BamI cleavage sites have been found and localized on the physical map of T5 DNA at 0.225; 0.68; 0.715 and 0.725 fractional lenght. Endonuclease PstI cuts T5 DNA at 11 sites: 0.095; 0.215; 0.320; 0.510; 0.635; 0.675; 0.710; 0.770; 0.810; 0.840; 0.875 fractional length. Six KpnI cleavage sites have been mapped at 0.140; 0.160; 0.530; 0.720; 0.760; 0.840 fractional length. 14 out of 17 HindIII cleavage sites have been localized at 0.10; 0.24; 0.255; 0.345; 0.40; 0.52; 0.54; 0.57; 0.70; 0.76; 0.80; 0.84; 0.87; 0.89, fractional length of T5 DNA. Complete cleavage map of the phage genome is presented for seven restriction enzymes.