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Updated: Aug 18, 2026

Non-Terminal Blood Sampling Techniques in Guinea Pigs
Published on: October 11, 2014
Effects of phenobarbital on digitoxin metabolism in guinea-pig liver slices
Abstract:
1. [3H]Digitoxin was incubated for 2-5 h with liver slices from control and phenobarbital-pretreated guinea-pigs. Metabolites were the same in both groups. 2. After 5 h incubation, a 6-fold increase in unmetabolized digitoxin was found in the phenobarbital-pretreated group compared with controls. 3. The major metabolite extracted by chloroform is formed by hydroxylation at an unknown position of the cardenolide nucleus, which is not the 12 beta-hydroxy position. Only half the amount of this metabolite was formed in livers of pretreated animals, compared to controls, in 2 h, after 5 h incubation similar amounts occurred in both groups. 4. Water-soluble metabolites decreased 2-fold in phenobarbital-pretreated animals at 2 and 5 h of incubation.
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