Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Hepatocyte changes in experimental iron overloading. An ultrastructural and microanalytic study]

A Lopez, J Vilches, L Bardet

    Annales D'Anatomie Pathologique
    |January 1, 1980
    PubMed
    Summary

    This study details ultrastructural changes in rat hepatocytes due to iron overload. Iron accumulation damages cellular organelles, including mitochondria and the endoplasmic reticulum, leading to cell injury.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Rectal drainage: unusual evolution of a psoas abscess.

    European journal of pediatric surgery : official journal of Austrian Association of Pediatric Surgery ... [et al] = Zeitschrift fur Kinderchirurgie·1998
    Same author

    Indolent systemic mast cell disease in adults: immunophenotypic characterization of bone marrow mast cells and its diagnostic implications.

    Blood·1998
    Same author

    Evaluation of the toxicity of guarana with in vitro bioassays.

    Ecotoxicology and environmental safety·1998
    Same author

    Skin necrosis at the injection site induced by low-molecular-weight heparin: case report and review.

    Dermatology (Basel, Switzerland)·1998
    Same author

    Severe hemolytic anemia due to multiple red cell alloantibodies after an ABO-incompatible allogeneic bone marrow transplant.

    Transfusion·1998
    Same author

    Rectocele is associated with paradoxical anal sphincter reaction.

    International journal of colorectal disease·1998

    Area of Science:

    • Hepatology
    • Cell Biology
    • Toxicology

    Background:

    • Iron overload can cause significant cellular damage in the liver.
    • Understanding the ultrastructural effects of iron overload is crucial for diagnosing and managing iron-related liver diseases.

    Purpose of the Study:

    • To analyze the ultrastructural changes in hepatocytes induced by experimental iron overloading.
    • To investigate the intracellular distribution of iron and its correlation with cellular lesions.

    Main Methods:

    • Oral and parenteral iron administration to Sprague-Dawley rats fed a specific diet.
    • Transmission electron microscopy for ultrastructural analysis of hepatocytes.
    • Microanalytic study of cellular organelles and nucleus.

    Related Experiment Videos

    Main Results:

    • Mitochondrial changes included flocculation, vesiculation, and crystolysis.
    • The rough endoplasmic reticulum (R.E.G.) exhibited dilatation, degranulation, and fragmentation.
    • Lysosomes increased in number and contained ferric macromolecules; degenerative nuclear changes were observed.

    Conclusions:

    • Experimental iron overload induces significant ultrastructural damage to rat hepatocytes.
    • Iron accumulation affects mitochondria, R.E.G., lysosomes, and the nucleus.
    • Microanalysis reveals a correlation between intracellular iron distribution and cellular injury.