[Quantitative study of biological denitrification in soil with the aid of acetylene. I.--Application on different...

The use of acetylene for investigation about biological denitrification in soils requires study of intensity and persistence of N2O-reductase activity inhibition by this gas and the effects on the other stages of denitrification and on the general metabolism of microflora. The methodology used is described, taking into account N2O solubility. N2O-evolution rates from nitrite and nitrate were measured during long-term incubations under acetylene, and complete inhibition of N2O-reductase was observed. After 2 weeks, acetylene inhibition appears decreased. The nitrate reduction rate seems increased in the presence of acetylene.