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Sequential nitrification by an Alcaligenes sp. and Nitrobacter agilis

D Castignetti, H B Gunner

    Canadian Journal of Microbiology
    |September 1, 1980
    PubMed
    Summary

    This study describes a soil bacterium, Alcaligenes sp., capable of high-level nitrification. When combined with Nitrobacter agilis, it forms a sequential nitrification system with ecological implications.

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    Area of Science:

    • Microbiology
    • Environmental Science
    • Biogeochemistry

    Background:

    • Assessing the ecological importance of heterotrophic nitrification is challenging due to typically low nitrification rates.
    • Heterotrophic nitrification, a process where organic compounds are converted to nitrite and nitrate by non-autotrophic bacteria, plays a role in nitrogen cycling.

    Purpose of the Study:

    • To describe a soil isolate, Alcaligenes sp., capable of significant nitrification.
    • To investigate the potential for a sequential nitrification system involving Alcaligenes sp. and a chemoautotroph.
    • To discuss the ecological significance of such a system.

    Main Methods:

    • Isolation and characterization of a soil bacterium, Alcaligenes sp.
    • Assaying nitrification activity by measuring nitrite production from pyruvic oxime.
    • Conducting co-culture experiments with Alcaligenes sp. and the chemoautotroph Nitrobacter agilis.
    • Monitoring nitrite and nitrate accumulation in batch cultures.

    Main Results:

    • The soil isolate, Alcaligenes sp., demonstrated high nitrification capacity, producing up to 1867 mg nitrite-nitrogen/L.
    • Co-culturing Alcaligenes sp. with Nitrobacter agilis resulted in sequential nitrification, with N. agilis consuming the nitrite produced by Alcaligenes sp.
    • Nitrate accumulation was observed in the sequential system, indicating efficient conversion of nitrite to nitrate.

    Conclusions:

    • Alcaligenes sp. represents a significant source of nitrification, potentially more impactful than previously recognized.
    • The identified sequential nitrification system highlights a microbial interaction with ecological relevance in nitrogen cycling.
    • Further research into such coupled nitrification processes can improve our understanding of nitrogen transformations in various ecosystems.

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