Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Scanning electron microscopy of human cerebellar cortex

O J Castejón, C J Valero

    Cell and Tissue Research
    |January 1, 1980
    PubMed
    Summary

    This study used scanning electron microscopy to reveal the intricate neuronal architecture and synaptic connections within the human cerebellum, detailing cell structures and their interactions.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Ultrastructural pathology of human peritumoural oedematous cerebellar cortex.

    Folia neuropathologica·2016
    Same author

    [Ultrastructural features of the synaptic plasticity in peritumoral cerebral oedema in humans].

    Revista de neurologia·2007
    Same author

    Nerve cell death types in the edematous human cerebral cortex.

    Journal of submicroscopic cytology and pathology·2007
    Same author

    The inflammatory reaction in human traumatic oedematous cerebral cortex.

    Journal of submicroscopic cytology and pathology·2005
    Same author

    Lysosome abnormalities and lipofucsin content of nerve cells of oedematous human cerebral cortex.

    Journal of submicroscopic cytology and pathology·2005
    Same author

    Nerve cell nuclear and nucleolar abnormalities in the human oedematous cerebral cortex. An electron microscopic study using cortical biopsies.

    Journal of submicroscopic cytology and pathology·2005

    Area of Science:

    • Neuroscience
    • Cell Biology
    • Microscopy

    Background:

    • The human cerebellum's complex neuronal circuitry is crucial for motor control and cognition.
    • Understanding its detailed architecture at the synaptic level is essential for neurological research.

    Purpose of the Study:

    • To investigate the three-dimensional neuronal architecture of the human cerebellum.
    • To characterize synaptic connections between cerebellar neurons using advanced microscopy.

    Main Methods:

    • Scanning electron microscopy (SEM) combined with cryofracture technique.
    • Processing samples using Humphreys et al. (1975) method with modifications.
    • Detailed imaging of granule cells, Golgi neurons, mossy fibers, climbing fibers, Purkinje cells, and glial cells.

    Main Results:

    • Granule cells possess unbranched axons and coniform dendrites forming "gearing type" synapses with mossy fiber rosettes.
    • Mossy rosettes appear as club-like structures; climbing fibers and Scheibel's collaterals were characterized.
    • Purkinje cells, basket cell axons, and parallel fibers were visualized in 3D, showing specific synaptic contacts.

    Conclusions:

    • SEM and cryofracture provide high-resolution insights into human cerebellar neuronal organization.
    • The study elucidates specific synaptic relationships, including granule cell-mossy fiber and parallel fiber-Purkinje cell interactions.
    • Detailed visualization aids in understanding cerebellar function and potential neuropathologies.

    Related Experiment Videos