Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Analysis for paraquat by second- and fourth-derivative spectroscopy

A F Fell, D R Jarvie, M J Stewart

    Clinical Chemistry
    |February 1, 1981
    PubMed
    Summary

    New derivative spectroscopy methods accurately detect paraquat in serum, plasma, and dialysis fluid. These precise techniques offer enhanced sensitivity for monitoring paraquat poisoning and assessing hemodialysis efficacy.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Impact of an automated report comparison tool on trainee report modification rate at a tertiary hospital.

    Clinical radiology·2024
    Same author

    Efficacy of detergent-based cleaning and wiping against SARS-CoV-2 on high-touch surfaces.

    Letters in applied microbiology·2023
    Same author

    Evaluation of surface disinfection methods to inactivate the beta coronavirus Murine Hepatitis Virus.

    Journal of occupational and environmental hygiene·2022
    Same author

    Nasal high-flow during neonatal and infant transport in Victoria, Australia.

    Acta paediatrica (Oslo, Norway : 1992)·2018
    Same author

    Silica in Relation to Pulmonary Disease.

    Edinburgh medical journal·2018
    Same author

    The effects of pregnancy on the pharmacokinetics of infliximab and adalimumab in inflammatory bowel disease.

    Alimentary pharmacology & therapeutics·2017

    Area of Science:

    • Analytical Chemistry
    • Clinical Chemistry

    Background:

    • Paraquat poisoning requires accurate and sensitive detection methods.
    • Existing methods may suffer from interference and limited sensitivity.

    Purpose of the Study:

    • To develop and validate simple, precise, and sensitive methods for paraquat analysis.
    • To enable routine monitoring of paraquat levels in clinical settings.

    Main Methods:

    • Utilized second and fourth-derivative spectroscopy.
    • Employed ion-pair extraction and dithionite reduction for plasma analysis.
    • Developed a rapid assay for dialysis fluid.

    Main Results:

    • Achieved a detection limit of 5 µg/L for plasma and 2 µg/L for dialysis fluid.
    • Demonstrated high precision (CV 2.7-5.2%) and accuracy.
    • Correlated well with radioimmunoassay (r=0.999) in poisoning cases.
    • Showed linear relationship from 10 to 1000 µg/L in plasma.

    Conclusions:

    • Derivative spectroscopy provides a sensitive and specific method for paraquat quantification.
    • Enables effective monitoring of paraquat levels during patient treatment.
    • Facilitates routine assessment of hemodialysis effectiveness.

    Related Experiment Videos