The synthesis of poly-L-lysine-succinyl-NADP: an analogue for NADP/H

Poly-L-lysine-succinyl-NADP, an analogue for nicotinamide adenine dinucleotide phosphate (NADP/H), has been synthesized by linking NADP/H to poly-L-lysine hydrobromide. This analogue (PL-SNP) was assayed with N, N dimethylaniline (DMA) in the presence of hepatic microsomal oxidase. A regeneration system of G-6-p and G-6-PD was used for reducing the PL-SNP+ to PL-SNPH. This reaction scheme with PL-SNP was found to exhibit up to 100% of the initial activity of pure NADP+ based on polarographic studies. Reaction rate of production for dimethylaniline oxide (DMA-0) was determined by oxygen consumption and by oxide formation. One of the potential uses of this analogue is as a cofactor in a membrane/liver enzyme detoxification system.