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Manganese effects on gluconeogenesis

R Rognstad

    The Journal of Biological Chemistry
    |February 25, 1981
    PubMed
    Summary
    This summary is machine-generated.

    Manganese (Mn2+) enhances the additive effects of glucagon and epinephrine on gluconeogenesis in rat hepatocytes. This suggests Mn2+ may stimulate key enzymes involved in glucose production.

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    Area of Science:

    • Biochemistry
    • Cellular Metabolism
    • Hormone Action

    Background:

    • Gluconeogenesis is a vital metabolic pathway for glucose homeostasis.
    • Hormones like glucagon and epinephrine regulate gluconeogenesis.
    • The role of manganese (Mn2+) in modulating this process is not fully understood.

    Purpose of the Study:

    • To investigate the effect of manganese (Mn2+) on gluconeogenesis in rat hepatocytes.
    • To determine if Mn2+ influences the additive effects of glucagon and epinephrine on glucose production.
    • To explore the potential enzymatic mechanisms underlying Mn2+ action.

    Main Methods:

    • Primary rat hepatocytes were isolated from fasted rats.
    • Gluconeogenesis was measured using lactate and pyruvate as substrates.

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  • The effects of glucagon, epinephrine, and manganese (Mn2+) were assessed individually and in combination.
  • Enzyme activity was probed using isotopic labeling with NaH14CO3.

    • Main Results:

    • Manganese (Mn2+) did not significantly increase basal gluconeogenesis from lactate/pyruvate.
    • Glucagon and epinephrine individually stimulated gluconeogenesis, but their effects were not additive without Mn2+.
    • In the presence of Mn2+, the hormonal effects became nearly completely additive.
    • Mn2+ increased the specific activity of glucose formed from lactate and NaH14CO3 by approximately 20%.

    Conclusions:

    • Manganese (Mn2+) potentiates the additive effects of glucagon and epinephrine on gluconeogenesis in rat hepatocytes.
    • The observed increase in specific activity suggests Mn2+ may stimulate pyruvate carboxylase or phosphoenolypyruvate carboxykinase.
    • These findings indicate a potential regulatory role for Mn2+ in key gluconeogenic enzymes, possibly by shifting them towards equilibrium.