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Related Experiment Videos

Reader error in determining minimal inhibitory concentrations with microdilution susceptibility test panels

A L Barry, L E Braun

    Journal of Clinical Microbiology
    |January 1, 1981
    PubMed
    Summary

    Reader agreement for microdilution susceptibility testing is high. Most minimal inhibitory concentration (MIC) readings showed excellent concordance between technologists, ensuring reliable antimicrobial susceptibility testing results.

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    Area of Science:

    • Microbiology
    • Clinical Laboratory Science
    • Antimicrobial Resistance

    Background:

    • Accurate antimicrobial susceptibility testing (AST) is crucial for guiding patient treatment and monitoring resistance.
    • Microdilution methods are standard for determining minimal inhibitory concentrations (MICs).
    • Inter-reader variability can impact the accuracy of MIC interpretations.

    Purpose of the Study:

    • To evaluate the inter-reader agreement for microdilution susceptibility test panels.
    • To quantify the rate of discrepancies in minimal inhibitory concentration (MIC) readings.
    • To assess the clinical significance of observed reading variations.

    Main Methods:

    • Two independent technologists read a large set of microdilution susceptibility test panels.

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  • A total of 25,022 minimal inhibitory concentrations (MICs) were recorded.
  • Discrepancies between readers were analyzed, with a focus on variations of two or more dilution intervals.

    • Main Results:

    • One or more discrepancies between readers occurred in 5% of the recorded MICs.
    • A significantly lower rate of 0.4% of tests showed a variation of greater than or equal to two dilution intervals.
    • The high concordance suggests robust methodology and technician training.

    Conclusions:

    • The microdilution susceptibility testing method demonstrates high inter-reader reliability.
    • Minor discrepancies are infrequent and rarely clinically significant (≥2 dilutions).
    • This supports the use of microdilution panels for routine antimicrobial susceptibility testing.