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Oxidative iodine monochloride iodination technique
The iodine monochloride (IC1) technique is used to radiolabel proteins under mild experimental conditions. Proteins labeled by this technique have been shown to have both in vitro in vivo characteristics often superior to those proteins labeled by the more frequently used chloramine-T method. Although first published more than two decades ago, use of the IC1 technique has largely been superceded by other more recently developed iodination methods. This paper attempts to reintroduce th oxidative IC1 method to the research community. This is accomplished by the presentation of a detailed protocol for a convenient modification of the original technique. A review of the qualities of the iodinated proteins produced by the IC1 and other iodination methods is also discussed.
The iodine monochloride (IC1) technique is used to radiolabel proteins under mild experimental conditions. Proteins labeled by this technique have been shown to have both in vitro in vivo characteristics often superior to those proteins labeled by the more frequently used chloramine-T method. Although first published more than two decades ago, use of the IC1 technique has largely been superceded by other more recently developed iodination methods. This paper attempts to reintroduce th oxidative IC1 method to the research community. This is accomplished by the presentation of a detailed protocol for a convenient modification of the original technique. A review of the qualities of the iodinated proteins produced by the IC1 and other iodination methods is also discussed.