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Related Experiment Videos

Studies on lipase in rat brain

M Mizobuchi, K Shirai, N Matsuoka

    Journal of Neurochemistry
    |January 1, 1981
    PubMed
    Summary

    This study measured lipase activity in rat brain homogenates, identifying two pH optima around 5.5 and 7.5. The findings suggest lipase activity produces free fatty acids and glycerol.

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    Area of Science:

    • Biochemistry
    • Neuroscience
    • Enzymology

    Background:

    • Lipases are crucial enzymes involved in lipid metabolism.
    • Understanding lipase activity in the central nervous system is vital for neurological research.

    Purpose of the Study:

    • To characterize lipase activity in rat cerebral hemispheres.
    • To determine the pH optima for lipase activity in this brain region.

    Main Methods:

    • Lipase activity was assessed using radioactive glycerol trioleate emulsified with Triton X-100.
    • Separation of labeled oleic acid was performed using a methanol-chloroform-heptane solvent mixture.
    • Assays were conducted on homogenates of rat cerebral hemispheres.

    Main Results:

    • Lipase activity exhibited dual pH optima, with peaks observed at approximately pH 5.5 and pH 7.5.
    • The enzymatic activity was quantified by measuring the release of labeled oleic acid.

    Conclusions:

    • Rat cerebral hemispheres possess lipase activity with distinct pH preferences.
    • The study suggests that free fatty acid and glycerol are the primary products of this lipase activity.

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