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Suppression of endogenous hydrocortisone with dexamethasone
Journal of Pharmaceutical Sciences
|December 1, 1980
Summary
This study found that dexamethasone effectively suppresses hydrocortisone. Treatments B (2 mg) and C (1 mg twice daily) provided adequate suppression for bioavailability studies.
Area of Science:
- Pharmacology
- Endocrinology
- Analytical Chemistry
Background:
- Endogenous hydrocortisone levels can interfere with drug bioavailability studies.
- Dexamethasone is a synthetic glucocorticoid often used for suppression testing.
Purpose of the Study:
- To determine the optimal dexamethasone dosage regimen for suppressing endogenous hydrocortisone.
- To assess the efficacy of different dexamethasone administration schedules.
Main Methods:
- A novel high-pressure liquid chromatography (HPLC) method was employed for precise quantification.
- Nine healthy volunteers participated in a randomized controlled trial with three treatment groups.
- Blood samples were collected pre- and post-dexamethasone administration to measure hydrocortisone levels.
Main Results:
- All tested dexamethasone regimens (A, B, and C) successfully suppressed endogenous hydrocortisone.
- Treatment B (2 mg at 11 pm) and Treatment C (1 mg at 11 pm and 1 mg at 6 am) demonstrated adequate and sustained suppression.
- Variations in the duration and extent of hydrocortisone suppression were observed across groups.
Conclusions:
- Dexamethasone is effective in suppressing endogenous hydrocortisone for bioavailability assessments.
- Regimens B and C are recommended for achieving adequate hydrocortisone suppression in clinical studies.
- The developed HPLC method provides a reliable tool for monitoring hydrocortisone levels.