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Updated: Aug 17, 2026

A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites
Published on: March 1, 2018
Khellin determination in human serum and urine by high-performance liquid chromatography
Abstract:
A rapid, sensitive, and specific high-performance liquid chromatographic method was developed for the determination of khellin in serum and urine using theophylline as the internal standard. Chromatography was performed on a reversed-phase C18 column with 65% (v/v) methanol as the mobile phase. The assay is capable of measuring khellin concentrations down to 100 ng/ml with a total procedure time of less than 20 min. Oral administration of 60 mg of khellin tablets to two healthy human males was carried out, and the serum samples were analyzed by the described method. Serum concentration-time profiles are presented.
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