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Related Experiment Videos

A human quantitative polymorphism related to Xg blood groups

P N Goodfellow, P Tippett

    Nature
    |January 29, 1981
    PubMed
    Summary

    A new monoclonal antibody, 12E7, identifies an antigen on human thymocytes. Its expression is X-linked and influenced by the Xg blood group phenotype, suggesting a sex-limited effect.

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    Area of Science:

    • Immunology
    • Human Genetics
    • Cell Biology

    Background:

    • Monoclonal antibody 12E7 targets an antigen expressed on human cortical thymocytes and other cells.
    • The 12E7 antigen shows species specificity, absent in mouse and hamster cells.
    • This specificity enables genetic studies using human-rodent somatic cell hybrids.

    Purpose of the Study:

    • To investigate the genetic basis of 12E7 antigen expression.
    • To determine the chromosomal linkage of the gene(s) controlling 12E7 antigen.
    • To explore a potential relationship between the 12E7 antigen and the Xg blood group system.

    Main Methods:

    • Generation of human-rodent somatic cell hybrids.
    • Analysis of 12E7 antigen expression in hybrid cells.
    • Comparison of 12E7 antigen expression with Xg blood group phenotypes.

    Main Results:

    • Hybrid cells retaining the human X chromosome expressed the 12E7 antigen.
    • This indicates X-linkage for the gene(s) governing 12E7 antigen expression.
    • A sex-limited effect of the Xg phenotype on 12E7 antigen expression was observed.

    Conclusions:

    • The 12E7 antigen expression is controlled by gene(s) located on the human X chromosome.
    • While distinct from Xga, the 12E7 antigen exhibits a sex-limited modulation by the Xg phenotype.
    • This finding highlights complex genetic interactions influencing cell surface antigen expression.

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