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Reactivity of hybridoma antibodies with amniotic and plasma fibronectin

Mouse antibodies were prepared to human amniotic fluid fibronectin by means of somatic cell hybrids. Comparison of immunological reactivity of antibodies with amniotic and plasma fibronectin revealed that the two molecules had very similar patterns of antigenic determinants. Antibodies from one cell population, however, bound better to amniotic fluid fibronectin, indicating that there is a difference in the molecular structure of fibronectins isolated from the two sources.

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