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In order to determine the residual heparin in the supernatant fluid recovered from blood washed with increasing amounts of solution in the Haemonetics Cell Saver, various modifications of Grann's polybrene neutralization test applied. It is suggested that each 450 to 500 ml of recovered heparinized blood be washed with a minimum of 500 ml of saline, although 700 ml is advisable. The red color from hemolyzed erythrocytes may clear while unacceptably high levels of heparin remain.
In order to determine the residual heparin in the supernatant fluid recovered from blood washed with increasing amounts of solution in the Haemonetics Cell Saver, various modifications of Grann's polybrene neutralization test applied. It is suggested that each 450 to 500 ml of recovered heparinized blood be washed with a minimum of 500 ml of saline, although 700 ml is advisable. The red color from hemolyzed erythrocytes may clear while unacceptably high levels of heparin remain.