Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A simple method for freezing lymphocytes in microtest plates

A S Goldstein, M C Hubbard, J M Barry

    American Journal of Clinical Pathology
    |February 1, 1981
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A cross-sectional, face-to-face, community survey of mental health and deprivation in post-recession Ireland: a rising tide lifts all boats?

    Irish journal of psychological medicine·2022
    Same author

    Community-based, cross-sectional study of self-reported health in post-recession Ireland: what has changed?

    QJM : monthly journal of the Association of Physicians·2018
    Same author

    The evolving role of axillary lymph node dissection in the modern era of breast cancer management.

    Surgical oncology·2011
    Same author

    Tunbridge Wells Infirmary and Dispensary.

    British medical journal·2010
    Same author

    Three Cases of Sudden Death.

    British medical journal·2010
    Same author

    Epithelial stem cells of the prostate and their role in cancer progression.

    Cold Spring Harbor symposia on quantitative biology·2008
    Same journal

    Invasive urothelial carcinoma in association with surface low-grade papillary urothelial carcinoma: clinical and pathologic insights from a rare entity.

    American journal of clinical pathology·2026
    Same journal

    CEACAM1 expression by immunohistochemistry in B-cell lymphomas and plasma cell myeloma.

    American journal of clinical pathology·2026
    Same journal

    Comprehensive multicriteria life cycle assessment of biopsy processing in a surgical pathology department.

    American journal of clinical pathology·2026
    Same journal

    Prognostic significance of Myb protein and its downstream target genes in lacrimal gland adenoid cystic carcinoma.

    American journal of clinical pathology·2026
    Same journal

    Mismatch repair protein "nonclassic expression loss" pattern in colorectal cancer: an important staining pattern that is not well understood.

    American journal of clinical pathology·2026
    Same journal

    Musculoskeletal pain in medical laboratory personnel: a cross-sectional study.

    American journal of clinical pathology·2026
    See all related articles

    This study introduces a cost-effective method for freezing lymphocytes in microtest plates, simplifying HLA antibody screening and reducing cell wastage. This technique offers significant time and cost savings for researchers.

    Area of Science:

    • Immunology
    • Cell Biology
    • Biotechnology

    Background:

    • Traditional methods for freezing lymphocytes for antibody screening are time-consuming and prone to errors.
    • Existing freezing techniques can lead to significant cell wastage and plate-loading inaccuracies.
    • High costs associated with commercial freezing units limit accessibility for many laboratories.

    Purpose of the Study:

    • To develop and describe a simple, reproducible, and inexpensive method for freezing lymphocytes in microtest plates.
    • To optimize the process for large-scale serum screening for HLA and other cytotoxic antibodies.
    • To offer a cost-effective alternative to commercial freezing units.

    Main Methods:

    • Cryoprotected lymphocytes were dispensed into microtest plates.

    Related Experiment Videos

  • Plates were placed on a metal table lowered into liquid nitrogen within a Styrofoam box.
  • Freezing rate was controlled by the liquid nitrogen depth and table height.
  • Over 100 microtest plates were frozen per cycle with uniform cooling.

    • Main Results:

    • The described method minimizes plate-loading errors and cell wastage.
    • Significant reduction in time required for serum screening.
    • Achieved total cooling uniformity across over 100 microtest plates per cycle.
    • The freezing device was assembled for under $400, a fraction of commercial unit costs.

    Conclusions:

    • Freezing lymphocytes in microtest plates is a viable and advantageous alternative to traditional vial-based methods.
    • This technique offers technical benefits including reduced errors, minimized cell loss, and increased efficiency.
    • The developed low-cost device provides an accessible solution for laboratories performing HLA and cytotoxic antibody screening.