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Blastocoel formation in half and double mouse embryos

M S Fernández, L Izquierdo

Anatomy and Embryology

|January 1, 1980

Summary

Peripheral sealing of the embryo precedes blastocoel formation, suggesting a causal link. Blastocoel development appears dependent on time post-fertilization, not cell count.

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Area of Science:

Developmental biology
Cell biology
Embryology

Background:

The formation of the blastocoel, a fluid-filled cavity, is a critical event in early mammalian embryonic development.
The precise mechanisms and triggers initiating blastocoel formation remain incompletely understood.
A proposed hypothesis links blastocoel formation to the peripheral sealing of the embryo.

Purpose of the Study:

To investigate the causal relationship between embryonic peripheral sealing and blastocoel formation.
To determine the role of cell number and cell cycle progression in blastocoel appearance.
To explore the influence of fertilization timing on blastocoel development.

Main Methods:

Utilizing electron microscopy to observe lanthanum tracer diffusion in mouse embryos.
Experimenting with manipulated embryo sizes (half and double embryos) alongside controls.
Quantifying cell numbers at the morula-blastocyst transition stage.

Main Results:

Peripheral sealing consistently preceded blastocoel appearance in all experimental groups and controls.
The total cell number and cell cycle count did not strongly correlate with blastocoel formation.
Experimental manipulations affected the rate of embryonic cleavage.

Conclusions:

The findings support a causal relationship where peripheral sealing initiates blastocoel formation.
Blastocoel development appears to be primarily time-dependent from fertilization.
Embryonic manipulation can alter cleavage rates, impacting developmental timing.

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