Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Structural study of Mycoplasma by a scanning electron microscopy method]

N D Konstantinova, I V Rakovskaia, L N Kats

    Zhurnal Mikrobiologii, Epidemiologii I Immunobiologii
    |September 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [Etiologic deciphering of community-acquired pneumonia caused by mycoplasma pneumoniae].

    Zhurnal mikrobiologii, epidemiologii i immunobiologii·2015
    Same author

    [Measurement of genital mycoplasma concentration by using a microbiological and molecular-biological methods].

    Zhurnal mikrobiologii, epidemiologii i immunobiologii·2014
    Same author

    [Polyphosphates in chlortetracycline producing Actinomyces aureofaciens].

    Antibiotiki·2014
    Same author

    [Effect of low temperature plasma on various mycoplasma species].

    Zhurnal mikrobiologii, epidemiologii i immunobiologii·2013
    Same author

    [Generalized mycoplasma infection in patients and carriers].

    Zhurnal mikrobiologii, epidemiologii i immunobiologii·2013
    Same author

    [Circulating immune complexes as a depot of conservation of mycoplasma cell components].

    Zhurnal mikrobiologii, epidemiologii i immunobiologii·2013

    Scanning electron microscopy revealed distinct structures in Mycoplasma arthritidis, Mycoplasma gallisepticum, and Acholeplasma laidlawii colonies. These colonies feature a framework of small spheres housing larger cells, rods, filaments, and large bodies.

    Area of Science:

    • Microbiology
    • Cell Biology
    • Microscopy

    Background:

    • Mycoplasma species are significant pathogens.
    • Understanding their morphology is crucial for diagnostics and treatment.
    • Previous studies have utilized various microscopy techniques.

    Purpose of the Study:

    • To investigate the ultrastructure of Mycoplasma arthritidis, Mycoplasma gallisepticum, and Acholeplasma laidlawii colonies.
    • To characterize the different morphological forms present within these colonies.
    • To compare the observed structures with known Mycoplasma and L-form characteristics.

    Main Methods:

    • Scanning electron microscopy (SEM) was employed.
    • Colonies of the specified Mycoplasma species were prepared for SEM analysis.

    Related Experiment Videos

  • High-resolution imaging was used to visualize cellular structures.

    • Main Results:

    • SEM revealed a reticular framework composed of small spherical elements (0.3–0.5 µm).
    • Larger spheroid cells (1–1.5 µm), irregular-shaped cells, rod-shaped (1–3 µm), and filamentous (3–5 µm) forms were observed within the framework.
    • Large bodies (5 x 3–8 x 3.5 µm), appearing as conglomerates of spheroid elements, were identified.
    • Small spheroid buds were present on the surface of all observed elements.

    Conclusions:

    • Mycoplasma colonies exhibit complex, multi-form structures.
    • The observed large bodies resemble those of L-forms, suggesting a potential relationship or developmental stage.
    • SEM provides valuable insights into the intricate morphology of these wall-less bacteria.