Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Correlations between deoxyribonucleic acid structural parameters and calculated circular dichroism spectra

B B Johnson, K S Dahl, I Tinoco

    Biochemistry
    |January 6, 1981
    PubMed
    Summary

    Circular dichroism (CD) spectra reveal how DNA helix winding and base pair twist influence spectral bands. This finding aids in understanding DNA structure through CD spectroscopy.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Distribution of Leaf-Feeding Beetles and Bean pod mottle virus (BPMV) in Illinois and Transmission of BPMV in Soybean.

    Plant disease·2019
    Same author

    Partitioning of variation derived from tissue culture of winter wheat.

    TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
    Same author

    Genetic analysis of a grass dwarf mutation induced by wheat callus culture.

    TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
    Same author

    DNA and RNA folds in transcription complex as evidenced by iodine-125 radioprobing.

    Journal of biomolecular structure & dynamics·2012
    Same author

    DNA bending and sugar switching.

    Journal of biomolecular structure & dynamics·2012
    Same author

    Biophysical analysis of nucleic acids.

    Current protocols in nucleic acid chemistry·2008

    Area of Science:

    • Biophysical Chemistry
    • Molecular Biology
    • Spectroscopy

    Background:

    • Circular dichroism (CD) spectroscopy is a powerful tool for analyzing DNA structure.
    • The CD spectrum of DNA is sensitive to its helical conformation.

    Purpose of the Study:

    • To investigate the relationship between DNA conformation and circular dichroism (CD) spectra.
    • To determine which conformational parameters best predict CD spectral features.

    Main Methods:

    • Computational calculation of CD spectra for 68 distinct DNA conformations.
    • Analysis of right-handed helical models, including published and energy-minimized forms.
    • Correlation analysis between CD spectral band magnitudes and geometric parameters.

    Main Results:

    Related Experiment Videos

    • The magnitude of the CD band at 274 nm shows a linear dependence on helix winding angle and base pair twist.
    • Poorer correlations were observed between the CD band magnitude and base pair distance, distance from the helix axis, or base pair tilt.

    Conclusions:

    • Helix winding angle and base pair twist are key determinants of the 274 nm CD band in DNA.
    • CD spectroscopy can effectively report on specific aspects of DNA helical geometry.