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Esterolytic method for determination of heparin in plasma
Clinical Chemistry
|April 1, 1981
Summary
This study presents a new heparin assay utilizing alpha-thrombin III acceleration. The assay accurately measures plasma heparin levels, showing good correlation with existing methods and activated partial thromboplastin times.
Area of Science:
- Biochemistry
- Clinical Chemistry
- Hematology
Background:
- Heparin monitoring is crucial for anticoagulant therapy.
- Existing heparin assays may have limitations.
- A reliable and precise heparin assay is needed.
Purpose of the Study:
- To develop and validate a novel heparin assay.
- To assess the assay's performance using automated analyzers.
- To compare the novel assay with established methods.
Main Methods:
- The assay measures the heparin-accelerated rate of alpha-thrombin III.
- Product formation is inversely proportional to plasma heparin concentration.
- Results were obtained using the discrete analyzer ABA-100.
- Assay precision was evaluated (CVs < 10%).
Main Results:
- The assay demonstrated good precision with coefficients of variation under 10%.
- A high correlation (r=0.90) was observed when compared to the Dade Protopath heparin assay.
- A moderate correlation (r=0.67) was found between the assay and activated partial thromboplastin times.
Conclusions:
- The developed heparin assay is precise and reliable.
- It shows excellent agreement with a commercial heparin assay.
- The assay is insensitive to antithrombin III concentrations, offering an advantage.