Bioassay of clindamycin in bile

This study compares different preparations of standard solutions for determining clindamycin in bile. Pooled human serum, ox bile, 10% human bile, and 0.1 M phosphate buffer were compared, and pooled human bile was serving as reference standard curve. The use of pooled human serum and ox bile resulted in a considerable error of overestimation of clindamycin in bile. An error of underestimation occurred when 10% human bile or 0.1 M phosphate buffer was used as a standard solution. It is concluded that none of the different preparations tested are acceptable substitutes for human bile.