Related Experiment Video
Updated: Aug 26, 2026

Hydraulic Extrusion of the Spinal Cord and Isolation of Dorsal Root Ganglia in Rodents
Published on: January 22, 2017
Spreading depression in isolated spinal cord
D S Streit1, C R Ferreira Filho, H Martins-Ferreira
1Faculdade de Medicina de Petrópolis, Instituto Biomédico, Universidde Federal Fluminense, R J, Brazil.
Abstract:
1. Isolated spinal cord of amphibians is able to express Leão's spreading depression (SD). 2. SD can be evoked when the spinal cord is in an appropriated medium. There are two conditions that favor its elicitation: hypotonicity and low [Cl-] levels in the extracellular microenvironment. 3. Spinal cord SD is accompanied by a stereotyped negative extracellular voltage transient of 17.3 +/- 4.9 (standard deviation) mV amplitude and 1.2 +/- 0.5 min duration concomitantly with a reversible [K+]o increase up to 21.1 +/- 4.6 mM in the extracellular fluid. 4. Potassium salt solution can trigger SD waves that spread at 12 +/- 4.7 mm/min. 5. SD waves occur "spontaneously" in preparations superfused by Ringer solutions with very low [Cl-] (< 20 mM). The frequency of these waves decreases, and they may stop if [Mg2+] is increased.
More Related Videos
Related Concept Videos
Long-term Depression
Long-term Depression
Calcium Ion Concentration Mechanism
If over time, all...
Spinal Cord Injury ll: Pathophysiology
Secondary Spinal Cord Injury llI: Pathophysiology

