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The properties of the multicopy suppressor of the ogd1 mutation in yeast
Y Gbelská1, D Mockovciaková, J Timko
1Comenius University, Department of Microbiology and Virology, Bratislava, Slovakia.
Journal of Basic Microbiology
|January 1, 1995
Abstract:
The 8.1 kb chromosomal fragment partially suppressing the ogd1 mutation in Saccharomyces cerevisiae has been cloned. The molecular analysis revealed that its suppressor gene codes for a natural glutamine tRNA(CAG) and maps on chromosome XIII in the upstream region of the URA10 gene. The multicopy plasmids containing this tRNA gene also suppressed the standard trp1-1 amber mutation and conferred the sensitivity of yeast cells to paromomycin and increased temperature.