[Enzyme immunoassay of blood coagulation and fibrinolysis factors]

Various types of immunoassays are at present applied to inspection items in clinical aspect of blood coagulation and fibrinolysis, in which EIA is acting as one of the major methods. Commercial EIA kits, available in Japan, can be itemized into categories of blood coagulation, fibrinolysis, endothelial cell injury, and platelet activation systems, along with other types of immunoassays and enzymatic activity assays, itemized for comparison. Assay methods suitable for an objective antigen should be selected, based on its biological, biochemical characteristics, such as plasma concentrations, and even clinical demands. The matters to be solved range over making reliable standard materials, using monoclonal antibody to detect absolute concentrates of specific antigen, and then further discovery of molecular markers that specifically reflect coagulative or fibrinolytic activation, leading to thrombotic or bleeding tendency.