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GTP-binding protein-mediated production of superoxide anion in rabbit gastric parietal cells
1Department of Pharmaceutical Physiology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Japan.
The Japanese Journal of Physiology
|January 1, 1995
Abstract:
We found that guanosine-5'-O-(3-thiotriphosphate) (GTP gamma S) increased superoxide production in the suspension rich in rabbit gastric parietal cells that were permeabilized by streptolysin O. In this experiment, oxidation of non-fluorescent dihydrorhodamine 123 into the fluorescent rhodamine 123 by oxygen radicals was measured. Results support that GTP gamma S closes a housekeeping C1- channel via intracellular production of superoxide anion in the parietal cell.