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Association of apolipoprotein E with murine amyloid A protein amyloid
1Department of Biochemistry, University of Kentucky School of Medicine, Lexington, USA.
Background:
Experimental amyloid A protein (AA) amyloidosis in mice is the most rapid type of amyloid formed, thus providing a valuable model to study amyloid formation. Recent studies have suggested the importance of apolipoprotein E (apoE) in Alzheimer's disease and systemic amyloidoses. To help understand the role of apoE in amyloidoses, we examined amyloid tissue for the presence of apoE in mouse AA amyloid.
Experimental Design:
Mice were injected with amyloid-enhancing factor and silver nitrate to induce amyloid deposition. Spleens were examined for Congo red staining and serum amyloid A, serum amyloid P component, and apoE immunostaining. In addition, RNA analysis was performed to measure the expression of apoE in various tissues after amyloid induction.
Results:
We have found that apoE is associated with mouse amyloid. Ab to apoE consistently detected the presence of increased levels of apoE in amyloid tissue. Immunohistochemical analyses confirmed that the apoE immunoreactivity co-associated with AA and serum amyloid P in the amyloid fibrils. Northern blot analysis of amyloid tissue showed an increase in apoE messenger RNA compared with control tissue.
Conclusions:
This is the first demonstration of apoE in mouse amyloid tissue. The data presented here, along with previous studies, suggest that apoE may be involved in amyloidogenesis. These studies validate the mouse model for studying the role of apoE in amyloid fibrillogenesis. The use of transgenic and gene-inactivated mice will help to elucidate the role and mechanism of apoE in amyloid formation.
Insights
Apolipoprotein E (apoE) is present in mouse amyloid A protein (AA) amyloid tissue, with increased apoE messenger RNA detected. These findings suggest apoE plays a role in amyloidogenesis and validate the mouse model for further study.
Area of Science:
- Biochemistry
- Molecular Biology
- Pathology
Background:
- Experimental amyloid A protein (AA) amyloidosis in mice serves as a rapid model for studying amyloid formation.
- Apolipoprotein E (apoE) has been implicated in Alzheimer's disease and systemic amyloidoses.
- Understanding apoE's role in amyloidogenesis is crucial for developing therapeutic strategies.
Purpose of the Study:
- To investigate the presence and role of apolipoprotein E (apoE) in mouse AA amyloidosis.
- To determine if apoE levels are altered in amyloid tissue.
- To validate the mouse AA amyloid model for studying apoE's function in amyloid fibril formation.
Main Methods:
- Induction of AA amyloidosis in mice using amyloid-enhancing factor and silver nitrate.
- Immunohistochemical analysis of spleen tissue for apoE, AA, and serum amyloid P component.
- Northern blot analysis to quantify apoE messenger RNA expression in amyloidotic tissues.
Main Results:
- Apolipoprotein E (apoE) was detected in mouse AA amyloid tissue.
- Immunohistochemistry confirmed apoE co-localization with AA and serum amyloid P within amyloid fibrils.
- Increased apoE messenger RNA levels were observed in amyloid tissue compared to controls.
Conclusions:
- This study provides the first evidence of apoE presence in mouse AA amyloid tissue.
- The findings suggest a potential role for apoE in the process of amyloidogenesis.
- The mouse AA amyloid model is validated for future research into apoE's mechanism in amyloid formation, including studies with transgenic and gene-inactivated mice.