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Related Experiment Videos

Leukemia cell derived factor (LDF) induces hematopoiesis

A Guha1, M Dibrino, M J Shields

  • 1Department of Medicine, University of Connecticut Health Center, Farmington 06030-1315, USA.

Leukemia
|October 1, 1995
PubMed
Summary
This summary is machine-generated.

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Certain leukemia cells stimulate red blood cell production by releasing a factor. Researchers identified this leukemia cell-derived factor (LDF) primarily associated with the cell membrane, potentially shed into the surrounding medium.

Area of Science:

  • Hematology
  • Cell Biology
  • Cancer Research

Background:

  • Acute lymphoblastic leukemia (ALL) is a cancer of early B-cell lineage.
  • Erythropoiesis, the production of red blood cells, is regulated by various factors.
  • Leukemic cells may influence normal hematopoietic processes.

Purpose of the Study:

  • To investigate if leukemic cells stimulate erythropoiesis.
  • To identify the specific leukemia cell-derived factor (LDF) responsible for this stimulation.
  • To determine the cellular localization and potential release mechanism of LDF.

Main Methods:

  • Co-culture of leukemic cell lines (A1, early B-cell ALL) and patient-derived B-cells with normal bone marrow cells.
  • Use of recombinant erythropoietin (rhEPO) in bioassays to assess burst forming unit erythroid (BFU-E) stimulation.

Related Experiment Videos

  • Cellular fractionation (lysis, differential centrifugation) to isolate membrane, cytosolic, and nuclear proteins.
  • Analysis of spent culture medium.
  • Ion-exchange Fast Protein Liquid Chromatography (FPLC) for protein separation.

    • Main Results:

    • Irradiated A1 cells stimulated BFU-E formation in the presence of rhEPO.
    • Not all tested leukemic cells induced BFU-E stimulation.
    • Leukemia cell-derived factor (LDF) activity was associated with plasma membrane proteins.
    • Spent medium from A1 cells also stimulated BFU-E, suggesting LDF shedding.
    • Ion-exchange FPLC identified specific fractions containing LDF activity.

    Conclusions:

    • Certain leukemic cells, like the A1 ALL cell line, can stimulate erythroid progenitor cells.
    • The stimulatory factor (LDF) appears to be a membrane-associated protein that may be shed.
    • Further characterization of LDF could reveal novel insights into leukemia-hematopoiesis interactions.