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Apoptin, a protein encoded by chicken anemia virus, induces cell death in various human hematologic malignant cells
S M Zhuang1, J E Landegent, C A Verschueren
1Laboratory of Molecular Carcinogenesis, Sylvius Laboratory, Leiden University, The Netherlands.
Abstract:
Apoptin, a small protein encoded by chicken anemia virus (CAV) was expressed in various human hematologic malignant cell lines derived from leukemias and lymphoma. Three of these cell lines contain bcl-2 or BCR-ABL proteins, known to block apoptosis induced by chemotherapeutic compounds. By immunofluorescence and propidium-iodide staining apoptin was shown to induce apoptosis in all analysed cell lines. Early after expression, apoptin exhibited a fine-granular distribution in the still intact nucleus. Later, apoptin became aggregated and the nucleus segmented. The data with truncated apoptin indicate that for optimal induction of apoptosis apoptin has to be located in the nucleus.