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Acute repeated nicotine injections increase enkephalin and decrease AP-1 DNA binding activity in rat adrenal medulla

M K McMillian1, P M Hudson, K L Simmons

  • 1Laboratory of Environmental Neurosciences, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA.

Brain Research. Molecular Brain Research
|July 1, 1995
PubMed
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Repeated acute nicotine injections significantly reduced AP-1 DNA binding activity in the adrenal medulla. This treatment also elevated adrenal tyrosine hydroxylase and [Met5]enkephalin levels in rats.

Area of Science:

  • Neuroscience
  • Pharmacology

Background:

  • Nicotine's effects on adrenal medulla function are complex.
  • Previous studies indicated nicotine alters AP-1 DNA binding activity.

Purpose of the Study:

  • To investigate the impact of repeated acute nicotine injections on adrenal medulla gene expression and DNA binding activity.
  • To examine the time course of these changes.

Main Methods:

  • Rats received repeated acute injections of nicotine (3 mg/kg i.p.) over a 3-hour period (7 injections).
  • Adrenal medulla AP-1 DNA binding activity, tyrosine hydroxylase, and [Met5]enkephalin levels were measured.

Main Results:

  • Nicotine injections profoundly decreased adrenal medulla AP-1 DNA binding activity for over 8 hours.

Related Experiment Videos

  • This treatment significantly increased adrenal tyrosine hydroxylase and [Met5]enkephalin levels.

    • Conclusions:

    • Repeated acute nicotine administration has a significant inhibitory effect on AP-1 DNA binding activity in the adrenal medulla.
    • Nicotine exposure modulates key markers of adrenal medulla function, including neurotransmitter synthesis and peptide levels.