Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Pro-sequence-assisted protein folding

J Eder1, A R Fersht

  • 1MRC Unit for Protein Function and Design, University Chemical Laboratory, Cambridge, UK.

Molecular Microbiology
|May 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

PACIC: patient activation and problem solving not related to age in patients with depressive symptoms.

European archives of psychiatry and clinical neuroscience·2025
Same author

Suboptimal antidepressant use among inpatients and outpatients with symptoms of depression: a cross-sectional analysis of the POKAL core data set.

European archives of psychiatry and clinical neuroscience·2025
Same author

[Weight gain and treatment with psychotropic drugs : Background and management].

Der Nervenarzt·2023
Same author

Predictors and outcomes in primary depression care (POKAL) - a research training group develops an innovative approach to collaborative care.

BMC primary care·2022
Same author

Impact of myocardial injury after coronary artery bypass grafting on long-term prognosis.

European heart journal·2022
Same author

Refractory lupus nephritis: a survey.

Lupus·2019
Same journal

Riboflavin Salvage Supports Glycolysis in Borrelia burgdorferi Through Flavin-Dependent NAD<sup>+</sup> Regeneration.

Molecular microbiology·2026
Same journal

Distinct Spatial Organisation of Rho and RNA Polymerase in Salmonella Cells.

Molecular microbiology·2026
Same journal

A Single-Nucleotide Substitution Generates a de Novo Promoter That Activates a Latent Metabolic Bypass in Escherichia coli.

Molecular microbiology·2026
Same journal

A Phosphorylation-Dependent Partner-Switching-Like Module Regulates a Glycosyltransferase Required for Heterocyst Polysaccharide Layer Formation in Anabaena sp. Strain PCC 7120.

Molecular microbiology·2026
Same journal

Chain-Length Regulation by WzzE Is Necessary for, but Genetically Separable From, Cyclic Enterobacterial Common Antigen Synthesis.

Molecular microbiology·2026
Same journal

To Move or Not to Move: When and How Bacteria Suppress Flagellar Motility.

Molecular microbiology·2026
See all related articles

Pro-sequences are crucial for protein folding and transport, acting directly to catalyze folding, unlike molecular chaperones. This suggests a common evolutionary mechanism for protein folding and secretion.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Protein Folding

Background:

  • Proteins like proteases and growth factors are synthesized as precursors called pre-pro-proteins.
  • Pre-sequences often function as signal peptides for cellular transport.
  • Pro-sequences are increasingly recognized for their essential role in the correct folding of associated proteins.

Purpose of the Study:

  • To investigate the role of pro-sequences in protein folding and intracellular transport.
  • To explore the catalytic mechanism of pro-sequences in protein folding.
  • To examine the evolutionary relationship between pro-sequence-assisted folding and protein secretion.

Main Methods:

  • Comparative analysis of pro-sequence-assisted folding mechanisms across different proteases.

Related Experiment Videos

  • Hypothesis testing regarding convergent evolution of protein folding.
  • Investigating the link between pro-sequence function in folding and transport/secretion.

    • Main Results:

    • Pro-sequences directly catalyze protein folding, distinct from molecular chaperone activity.
    • Similarities in folding mechanisms among proteases suggest convergent evolution.
    • Pro-sequences are frequently required for both protein folding and secretion.

    Conclusions:

    • Pro-sequences play a vital, direct role in catalyzing protein folding.
    • Convergent evolution likely shaped common pro-sequence-assisted folding mechanisms.
    • Protein folding and intracellular transport/secretion are intrinsically linked functions mediated by pro-sequences.