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Assessment of two protocols for the human lymphocyte cytokinesis-blocked micronucleus assay
T K Lee1, J Johnson, A L Wiley
1Department of Radiation Oncology, Leo W. Jenkins Cancer Center, East Carolina University, Greenville, NC 27858, USA.
Mutagenesis
|July 1, 1995
Abstract:
This study indicated that by adding cytochalasin B (6 micrograms/ml) at 24 h, the lymphocyte culture time for micronucleus (MN) assay could be shortened to 64 h. In both unirradiated and ex-vivo irradiated (2 Gy) lymphocytes from three populations, we found that the differences in MN yield obtained by our modified cytokinesis-blocked time frame and that recommended by Fenech and Morley (1985) were insignificant (P = 0.66-0.87). We believe that the shorter assay time may enhance the applicability of MN assay for the rapid assessment of ionizing radiation overexposures.