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[A polymorphism study of the DNA extracted from dental tissues]
G Dell'Osso1, M Avitabile, G Sciacca
1Istituto di Medicina Legale e delle Assicurazioni, Università degli Studi, Catania.
Minerva Stomatologica
|May 1, 1995
Summary
Forensic identification can utilize DNA extracted from teeth, even those up to five years old. This method amplifies variable number tandem repeat (VNTR) sequences for reliable personal identification.
Area of Science:
- Forensic Odontostomatology
- Molecular Biology
- Genetics
Context:
- Dental elements are crucial for personal identification in forensic medicine.
- Establishing reliable DNA profiles from aged or degraded dental samples presents challenges.
Purpose:
- To develop and validate a method for DNA extraction and amplification from human teeth.
- To assess the efficacy of Polymerase Chain Reaction (PCR) for amplifying VNTR sequences in dental DNA.
Summary:
- A novel method for extracting and amplifying DNA from teeth aged two weeks to five years post-avulsion is presented.
- Variable number tandem repeat (VNTR) sequences were amplified using PCR.
- Amplified products were visualized and compared to matching blood samples for verification.
Impact:
- This technique provides a powerful new tool for personal identification in forensic odontology.
- The method is effective for both recent and ancient teeth, enhancing forensic casework capabilities.