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Related Experiment Videos

Unitary anion currents through phospholemman channel molecules

J R Moorman1, S J Ackerman, G C Kowdley

  • 1Department of Internal Medicine (Cardiovascular Division), University of Virginia Health Sciences Center, Charlottesville 22908, USA.

Nature
|October 26, 1995
PubMed
Summary

Phospholemman (PLM) forms ion channels, making it the smallest known membrane protein to do so. This discovery suggests PLM

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Area of Science:

  • Membrane biophysics
  • Ion transport mechanisms
  • Molecular biology

Background:

  • Phospholemman (PLM) is a small membrane protein known to induce currents.
  • Its precise function as an ion channel or regulator remained unclear.

Purpose of the Study:

  • To determine if Phospholemman (PLM) functions as an ion channel.
  • To characterize the ion transport properties of PLM.

Main Methods:

  • Measuring unitary anion currents in planar lipid bilayers with purified recombinant PLM.
  • Analyzing currents in excised patches from oocytes expressing PLM.
  • Quantifying radiolabeled taurine flux in oocytes.

Main Results:

  • Recombinant PLM formed ion channels in planar lipid bilayers.

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  • Oocyte patches expressing PLM showed similar channel activity.
  • Taurine was highly permeant through PLM channels.
  • PLM expression enhanced taurine transport in oocytes.

    • Conclusions:

    • Phospholemman (PLM) is a functional ion channel, the smallest identified to date.
    • PLM's taurine selectivity points to a role in cell volume regulation.