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The viral oncoproteins Ad5 E1A, HPV16 E7 and SV40 TAg bind a common region of the TBP-associated factor-110

J M Mazzarelli1, G B Atkins, J V Geisberg

  • 1Department of Microbiology, School of Dental Medicine, University of Pennsylvania, Philadelphia 19104, USA.

Oncogene
|November 2, 1995
PubMed

Insights

Oncoproteins from adenovirus E1A, papillomavirus E7, and SV40 TAg bind to TBP-associated factor-110 (TAF-110). This interaction, occurring in the same TAF-110 region, suggests a role in cell growth regulation and viral pathogenesis.

Area of Science:

  • Molecular Biology
  • Virology
  • Cell Biology

Background:

  • Oncoproteins like adenovirus E1A, papillomavirus E7, and SV40 TAg disrupt normal cell growth.
  • These oncoproteins are known to interact with retinoblastoma protein family members.

Purpose of the Study:

  • To investigate the interaction between E1A, E7, and TAg oncoproteins and TBP-associated factor-110 (TAF-110).
  • To determine the specific binding region of TAF-110 for these oncoproteins.
  • To explore the functional implications of these interactions in viral pathogenesis.

Main Methods:

  • Protein binding assays were used to demonstrate interactions.
  • The carboxyl region of TAF-110 was identified as the binding site for the oncoproteins.

Main Results:

  • Adenovirus E1A, papillomavirus E7, and SV40 TAg oncoproteins all bind to TAF-110.
  • The binding occurs within the same 77 amino acid carboxyl region of TAF-110.
  • E1A and TAg binding to TAF-110 is consistent with their known roles in transcription initiation via TBP interactions.

Conclusions:

  • Oncoproteins E1A, E7, and TAg interact with TAF-110, suggesting a common mechanism for cell growth interference.
  • Papillomavirus E7's interaction with TAF-110, in addition to TBP, may contribute to human papillomavirus pathogenesis by modulating specific promoter expression.

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