Ontogeny of L-alanine uptake in plasma membrane vesicles from rat liver

Alanine uptake into liver plasma membrane vesicles was studied at different stages of postnatal rat development. Before weaning, alanine hepatic uptake showed lower values for the global KM than after weaning (0.34, 0.77, 1.45, and 1.61 mM for 1-, 15-, and 28-d-old and adult rats, respectively). Alanine uptake capacity increased progressively until reaching maximum values in the adult state (values for Vmax: 0.078, 0.199, 0.317, and 0.613 nmol alanine/mg protein/3 s for 1-, 15-, and 28-d-old and adult rats, respectively). These results seem to point to a prevalence of a high affinity, low capacity alanine transport component (traditionally assumed to be attributable to system A) in newborn and suckling rats, in agreement with our previous results on isolated hepatocytes (Martínez-Mas JV, Casado J, Felipe A, Marin JJG, Pastor-Anglada M: Biochem J 293: 819-824, 1993). The suckling-weaning developmental transition seems to play a role in establishing the pattern of adult hepatic alanine transport characterized by a higher capacity but a lower affinity (because most alanine is taken up by system ASC) inasmuch as KM values show a 100% increase after weaning, although Vmax values continue to increase steadily until the adult age.